生物体系中的化学测量

书籍目录

第一章 绪论第二章 水、pH值和缓冲液第三章 紫外及可见吸收分光光度法和光度测定法第四章 检测反应（比色反应）第五章 酶及其作为试剂的应用第六章 酶活性测定第七章 复杂混合物品样品测定的工具——色谱法第八章 电泳和其他电动分离第九章 酶联免疫吸附测定法第十章 分析方法的质量挖掘与数据确认第十一章 分析方法的选择附录一 单位附录二 生物学体系中化学分析的统计学附录三 标准曲线和线性回归分析附录四 稀释表

作者简介

Techniques in Analytical Chemistry How to evaluate and validate data from chemical measurements in biological systems-a unique overview An understanding of chemical measurement processes in biological systems is essential to working in today's life-science and biotechnology industries. This work provides analysts and laboratory professionals with the fundamental knowledge and basic principles they need to tackle the measurement process in the real world. Rather than rehashing traditional analytical techniques, which can be problematic for biological samples, the book emphasizes the highly complex nature of biological systems through such topics as enzyme measurements, enzymes as reagents, immunochemical assays, and assay validation and quality control. It provides a complete overview of principles and methods proven useful in biological systems-from pH, buffers, and spectrophotometry to basic chromatographic techniques. With Chemical Measurements in Biological Systems as their guide, readers will understand the basic principles needed to: Read and evaluate an assay method description Choose the appropriate method from the vast array of available techniques Modify and evaluate an existing method for specific needs Set up and run biochemical assays Use multistep problem-solving techniques Evaluate the results of a given assay in a given matrix

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