果蝇实验指南

书籍目录

PrefaceAbbreviationsCHROMOSOMES　1 Preparation and Analysis of Drosophila Mitotic Chromosomes　　2 In Situ Hybridization to Somatic Chromosomes  3 BrdU Labeling of Chromosomes  4 Analysis of Meiosis of Fixed and Live Oocytes by Light Microscopy  5 Cytological Analysis of Spermatocyte Growth and Male Meiosis in Drosophila melanogaster  6 Preparation and Analysis of Polytene Chromosomes  7 In Situ Hybridizaiton to Polytene Chromosomes  8 Mapping Protein Distributions on Polytene Chromosomes by ImmunostainingCELL BIOLOGY  9 Fluorescent Analysis of Drosophila Embryos  10 Imaginal Discs  11 The Neuromuscular Junction  12 Histological Techniques for the Drosophila Eye.Part Ⅰ:Larva and Pupa  13 Histological Techniques for the Drosophila Eye.Part Ⅱ:Adult  14 Preparation of Thin Sections of Drosophila for Examination by Transmission Electron Microscopy  15 Electrophysiological Approaches to the Neuromusculature  16 Functional Assays of the Peripheral and Central Nervous Systems  17 GFP and Other Reporters  18 Quantitative Microinjection of Drosophila Embryos  19 Targeted Disruption of Gene Function in Drosophila by RNA Interference  20 Drosophila Cell Culture and TRransformation  21 Generating Antibodies against Drosophila Proteins  22 Photoactivated Gene Expression for Cell-fate Mapping and Cell ManipulationMOLECULAR BIOLOGY  23 Recovery of DNA Sequences Flanking P-element Insertions:Inverse PCR and Plasmid Rescue  24 GAL4-mediated Ectopic Gene Expression in Drosophila  25 Functional Cell AblationGENOMICS  26 Preparation of DNA from E.coli Cells Containing Bacteriophage P1 Clones  27 Drosophila Microarrays:From Arrayer Construction to Hybridization  28 Using Drosophila Genome Databases  29 Preparation and Analysis of High-molecular-weight DNA in DrosophilaBIOCHEMISTRY  30 Culturing Large Populations of Drosophila for Protein Biochemistry  31 Preparation of Nuclear Extracts from Drosophila Embryos and In Vitro Transcription Analysis  32 Preparation of Membrane Proteins from Drosophila Embryos  33 Preparing Cytoplasmic Extracts from Drosophila Embryos  34 Immunoblotting of Proteins from Single Drosophila EmbryosTHE ORGANISM  35 Laboratory Culture of Drosophila  36 Preparation of Larval and Adult Cuticles for Light Microscopy  37 Exposing Drosophila to Neuroactive DrugsAPPENDICES  1 1989 Table of Contents from Drosophila:A Laboratory Manual  2 Anatomical Drawings of Drosophila  3 Solutions and Reagents  4 Cautions  5 Suppliers  6 TrademarksIndex

作者简介

《果蝇实验指南(影印版)》简介：果蝇是遗传学、发育学研究的重要模式生物之一，对于发育生物学、基因组学领域中的探索具有极为重要的意义。本书共提供了近40个未来10年内最可能用到的以果蝇为实验对象的分子生物学、生物化学和细胞生物学研究的实验方案。每一个方案都经过专家的精心挑选和雕琢，实验设计严谨、准确、简洁、规范，可操作性强，值得称道。方案涉及染色体、细胞、基因组和发育等方面，既包含了初学者需要了解的基础知识，也涵盖了资深研究者所需的细节。《果蝇实验指南(影印版)》的版式设计侧重于方便读者使用，正文中穿插了丰富的图表作为实验设计的辅助说明，附录中还列出了果蝇研究所需的重要资料如基本溶液、缓冲液的配方和配制方法等。

图书封面

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